Intron retention and nuclear loss of SFPQ are molecular …

The most significant IR is seen in the SFPQ transcript. The SFPQ protein binds extensively to its retained intron, exhibits lower nuclear abundance in VCP mutant cultures and is lost from nuclei of MNs in mouse models and human sporadic ALS. Collectively, we demonstrate SFPQ IR and nuclear loss as molecular hallmarks of …

Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 …

Bioinformatic analysis of SFPQ protein sequence and transport parameters of SFPQ granules in axons of DRG sensory neurons. (A) Schematic of protein domains of SFPQ and the probability score of the SFPQ sequence for being disordered and prion like as predicted by IUPred2 (top) and PLAAC (bottom), respectively.(B and C) Frequency …

The Splicing Factor Proline-Glutamine Rich (SFPQ/PSF) Is …

Figure S2: Dependence of SFPQ/PSF for the in vitro polyadenylation of transcripts. Recombinant RNPs were generated in control-silenced (C) or SFPQ/PSF-silenced (S2) HEK293T cells and in vitro transcription was performed as indicated in the legend to Figures 11 and and12. 12.The transcripts were separated into poly A + (blue) …

Arginine methylation and citrullination of splicing factor …

SFPQ and NONO are multifunctional nuclear proteins that appear to play a role in a diverse range of key processes within the cell including RNA processing, RNA splicing, and transcriptional regulation. Here we show that a number of N-terminal arginine residues in SFPQ are asymmetrically dimethylated in mammalian cells, a reaction that …

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EBV noncoding RNA EBER2 interacts with host RNA …

resin. SFPQ and NONO bound to MBP–Pax5, whereas RBM14 and AUF1, the negative control, did not (Fig. 3B). Taken together, these results argue that SFPQ and RBM14 make direct contacts with EBER2, whereas SFPQ and NONO interact specifically with PAX5 (Fig. 4D). Nuclear Extract 0.1 M Glycine pH 2 2.4 M TEACl ASO EBER2 Anti-PAX5 Affinity …

SFPQ Antibody | Cell Signaling Technology

Background. Splicing factor proline-glutamine rich (SFPQ), also commonly referred to as PTB-associated splicing factor (PSF), is an essential and ubiquitous nucleic acid-binding protein that is involved in a wide range of cellular processes. SFPQ belongs to the Drosophila melanogaster behavior, human splicing (DBHS) protein family along with ...

Post-transcriptional gene silencing mediated by microRNAs …

Here, we show by quantitative proteomic analysis that Ago2 interacts with the nucleoplasmic protein Sfpq in an RNA-dependent fashion. By a combination of HITS-CLIP and transcriptomic analyses, we demonstrate that Sfpq directly controls the miRNA targeting of a subset of binding sites by local binding. Sfpq modulates miRNA targeting …

SFPQ Promotes Lung Cancer Malignancy

Figure 2 The expression level of SFPQ in lung NSC cancer MSC is higher than that in IPF and control MSC.(A) Predicted interactive proteins of SFPQ and their functional interactions are shown by IPA pathway analysis. Primary cell lines were used to measure SFPQ expression level in MSCs. The MSCs were sorted and verified from 4 …

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We report an unexpected discovery of the infinite polymerization of SFPQ that is induced by zinc binding to the protein. The crystal structure of human SFPQ in …

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Altered Tau Isoform Ratio Caused by Loss of FUS and SFPQ …

Fused in sarcoma (FUS) and splicing factor, proline- and glutamine-rich (SFPQ) are RNA binding proteins that regulate RNA metabolism. We found that alternative splicing of the Mapt gene at exon 10, which generates 4-repeat tau (4R-T) and 3-repeat tau (3R-T), is regulated by interactions between FUS and SFPQ in the nuclei of neurons. …

FUNCTIONS OF SFPQ, NONO, AND NEAT1 RNA IN CELLS

Abstract About 20 years ago, large RNA–protein complexes called paraspeckles were discovered in cell nuclei. The main components of these complexes are SFPQ and NONO proteins and the long noncoding RNA NEAT1. Later, these proteins were found free in the nucleus and even in the cytoplasm. The functions of NEAT1 and …

SFPQ and NONO suppress RNA:DNA-hybrid-related …

NONO and SFPQ are TERRA interacting proteins. In order to identify novel TERRA interacting proteins, we performed TERRA RNA-pull-down experiments using mouse embryonic stem cells that maintain telomeres via telomerase dependent and independent telomere maintenance pathways 39.Biotinylated RNA oligonucleotides …

Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal …

Loss of Zebrafish sfpq Affects Development of Brain Boundaries and Motor Function. A recessive zebrafish mutant identified during a small-scale ENU mutagenesis screen was selected for its early brain boundary defects and was named coma due to its vegetative state and its later comma-shaped body (Figure S1A). Preceding any …

splicing-factor related protein SFPQ/PSF interacts with …

The transient nature of siRNA knockdown as well as low levels of protein that may still be present cannot entirely rule out the possibility that SFPQ might still be indispensible. Consistent with these results, an sfpq point mutation (Whitesnake) in zebra fish embryos did not affect cell proliferation, but increased cell death by ∼2-fold ( 54).

The RNA-binding protein SFPQ preserves long-intron …

SFPQ could help avoiding the use of premature cleavage and polyA sites, which are known to alter circRNA formation (Liang et al., 2017). The authors should discuss this in the context of the role of U1 snRNP on usage of intronic PAS (the telescripting mechanism uncovered by Dreyfuss and colleagues). The authors should also determine …

SFPQ Promotes Lung Cancer Malignancy via Regulation of …

SFPQ has been reported responsible for metastasis in colorectal cancer and nasopharyngeal carcinoma (22, 51). In vivo study, knockdown of SFPQ in NSC lung cancer-MSCs reduces their ability to metastasize distantly, which suggests that SFPQ is a potential therapeutic target for cancer metastasis, although clear mechanisms need to be …

SFPQ, a multifunctional nuclear protein, regulates the

In this study, we investigated the transcriptional regulation of PDE3A, and found that the splicing factor proline and glutamine rich (SFPQ) protein modulated PDE3A mRNA levels. Multiple transcription start sites (TSS1, 2, and 3) were identified within the first exon of PDE3A using 5'-rapid amplification of cDNA ends (RACE).

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UniProt

The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of …

Molecular Modelling of NONO and SFPQ Dimerization …

Abstract. NONO and SFPQ are involved in multiple nuclear processes (e.g., pre-mRNA splicing, DNA repair, and transcriptional regulation). These proteins, along with NEAT1, enable paraspeckle formation, thus promoting multiple myeloma cell survival. In this paper, we investigate NONO and SFPQ dimer stability, highlighting the hetero- and ...