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NEAT1_2 is the organizer of nuclear paraspeckles that sequester the RNA-binding proteins NONO and SFPQ. We show that upon oncogenic activation, mTORC1 …

The most significant IR is seen in the SFPQ transcript. The SFPQ protein binds extensively to its retained intron, exhibits lower nuclear abundance in VCP mutant cultures and is lost from nuclei of MNs in mouse models and human sporadic ALS. Collectively, we demonstrate SFPQ IR and nuclear loss as molecular hallmarks of …

In this report, we found that serum concentration regulated SFPQ binding to the PDE3A promoter and resulted in decreased PDE3A levels without any change in …

Bioinformatic analysis of SFPQ protein sequence and transport parameters of SFPQ granules in axons of DRG sensory neurons. (A) Schematic of protein domains of SFPQ and the probability score of the SFPQ sequence for being disordered and prion like as predicted by IUPred2 (top) and PLAAC (bottom), respectively.(B and C) Frequency …

Figure S2: Dependence of SFPQ/PSF for the in vitro polyadenylation of transcripts. Recombinant RNPs were generated in control-silenced (C) or SFPQ/PSF-silenced (S2) HEK293T cells and in vitro transcription was performed as indicated in the legend to Figures 11 and and12. 12.The transcripts were separated into poly A + (blue) …

A range of MS approaches were undertaken to comprehensively characterize and identify sites of arginine methylation in SFPQ including the use of both …

SFPQ and NONO are multifunctional nuclear proteins that appear to play a role in a diverse range of key processes within the cell including RNA processing, RNA splicing, and transcriptional regulation. Here we show that a number of N-terminal arginine residues in SFPQ are asymmetrically dimethylated in mammalian cells, a reaction that …

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The multifunctional protein, splicing factor, proline- and glutamine-rich (SFPQ) has been implicated in numerous cancers often due to interaction with coding and non …

resin. SFPQ and NONO bound to MBP–Pax5, whereas RBM14 and AUF1, the negative control, did not (Fig. 3B). Taken together, these results argue that SFPQ and RBM14 make direct contacts with EBER2, whereas SFPQ and NONO interact specifically with PAX5 (Fig. 4D). Nuclear Extract 0.1 M Glycine pH 2 2.4 M TEACl ASO EBER2 Anti-PAX5 Affinity …

The nuclear function of SFPQ. SFPQ is critical for the structural integrity of paraspeckles (A). SFPQ interacts with other …

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Background. Splicing factor proline-glutamine rich (SFPQ), also commonly referred to as PTB-associated splicing factor (PSF), is an essential and ubiquitous nucleic acid-binding protein that is involved in a wide range of cellular processes. SFPQ belongs to the Drosophila melanogaster behavior, human splicing (DBHS) protein family along with ...

Here, we show by quantitative proteomic analysis that Ago2 interacts with the nucleoplasmic protein Sfpq in an RNA-dependent fashion. By a combination of HITS-CLIP and transcriptomic analyses, we demonstrate that Sfpq directly controls the miRNA targeting of a subset of binding sites by local binding. Sfpq modulates miRNA targeting …

Crystals for SFPQ-276–598 were obtained from sitting-drop vapor method at 20°C using a reservoir solution of 26% 1,4-dioxane, 50 mM MOPS (pH 7.0), 5 mM MgCl 2 and 1 mM spermine. Six microliters protein (8.7 mg/ml) was mixed with 3 μl reservoir solution and equilibrated against 0.5 ml reservoir solution.

SFPQ N533H and L534I bind to zinc with higher affinities than the WT protein. (a) Schematic domain organization of human SFPQ, depicting the positions of the zinc-coordinating residues (H483, H528, and H530) and the reported fALS-associated mutations, N533H and L534I.(b) Crystal structure of human SFPQ with a close-up view …

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Figure 2 The expression level of SFPQ in lung NSC cancer MSC is higher than that in IPF and control MSC.(A) Predicted interactive proteins of SFPQ and their functional interactions are shown by IPA pathway analysis. Primary cell lines were used to measure SFPQ expression level in MSCs. The MSCs were sorted and verified from 4 …

The crystal structure of human SFPQ in complex with zinc at 1.94 Å resolution reveals intermolecular interactions between SFPQ molecules that are mediated by zinc. As anticipated from the crystal structure, the application of zinc to primary cortical neurons induced the cytoplasmic accumulation and aggregation of SFPQ.

We report an unexpected discovery of the infinite polymerization of SFPQ that is induced by zinc binding to the protein. The crystal structure of human SFPQ in …

The fact that NONO and SFPQ contain multiple RNA binding domains suggests an "RNA-based" role of NONO and SFPQ in …

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Fused in sarcoma (FUS) and splicing factor, proline- and glutamine-rich (SFPQ) are RNA binding proteins that regulate RNA metabolism. We found that alternative splicing of the Mapt gene at exon 10, which generates 4-repeat tau (4R-T) and 3-repeat tau (3R-T), is regulated by interactions between FUS and SFPQ in the nuclei of neurons. …

Abstract About 20 years ago, large RNA–protein complexes called paraspeckles were discovered in cell nuclei. The main components of these complexes are SFPQ and NONO proteins and the long noncoding RNA NEAT1. Later, these proteins were found free in the nucleus and even in the cytoplasm. The functions of NEAT1 and …

NONO and SFPQ are TERRA interacting proteins. In order to identify novel TERRA interacting proteins, we performed TERRA RNA-pull-down experiments using mouse embryonic stem cells that maintain telomeres via telomerase dependent and independent telomere maintenance pathways 39.Biotinylated RNA oligonucleotides …

Loss of Zebrafish sfpq Affects Development of Brain Boundaries and Motor Function. A recessive zebrafish mutant identified during a small-scale ENU mutagenesis screen was selected for its early brain boundary defects and was named coma due to its vegetative state and its later comma-shaped body (Figure S1A). Preceding any …

The transient nature of siRNA knockdown as well as low levels of protein that may still be present cannot entirely rule out the possibility that SFPQ might still be indispensible. Consistent with these results, an sfpq point mutation (Whitesnake) in zebra fish embryos did not affect cell proliferation, but increased cell death by ∼2-fold ( 54).

SFPQ could help avoiding the use of premature cleavage and polyA sites, which are known to alter circRNA formation (Liang et al., 2017). The authors should discuss this in the context of the role of U1 snRNP on usage of intronic PAS (the telescripting mechanism uncovered by Dreyfuss and colleagues). The authors should also determine …

SFPQ has been reported responsible for metastasis in colorectal cancer and nasopharyngeal carcinoma (22, 51). In vivo study, knockdown of SFPQ in NSC lung cancer-MSCs reduces their ability to metastasize distantly, which suggests that SFPQ is a potential therapeutic target for cancer metastasis, although clear mechanisms need to be …

In this study, we investigated the transcriptional regulation of PDE3A, and found that the splicing factor proline and glutamine rich (SFPQ) protein modulated PDE3A mRNA levels. Multiple transcription start sites (TSS1, 2, and 3) were identified within the first exon of PDE3A using 5'-rapid amplification of cDNA ends (RACE).

SFPQ ensures long-gene expression (HEK293T + MOUSE). (A) Volcano plot stratified by genes higher or lower than median gene length, where length is the …

The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. SFPQ isoform Long binds to the DNA binding …

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The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of …

Abstract. NONO and SFPQ are involved in multiple nuclear processes (e.g., pre-mRNA splicing, DNA repair, and transcriptional regulation). These proteins, along with NEAT1, enable paraspeckle formation, thus promoting multiple myeloma cell survival. In this paper, we investigate NONO and SFPQ dimer stability, highlighting the hetero- and ...

Splicing Factor Proline/Glutamine Rich (SFPQ) is a ubiquitously expressed RNA-binding protein of the DBHS family with diverse roles in alternative splicing, …